Figure 1.

General schematic of the inducible (small molecule) reverse two-hybrid system designed to detect small molecule inhibitors of protein–protein interactions. The expression of the interacting proteins is controlled by the GAL1 promoter, which is repressed in Glc. After Gal induction, the two interacting protein partners are synthesized, and their association in turn induces the synthesis of a toxic gene product, leading to death, unless an inhibitor of the protein–protein interaction is present in the cell. Only cells with this disruption should be selected.