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(A) Reporter constructs used for homologous recombination. The LexAop4–SPO13tata–URA3 reporter construct contains four LexA operators upstream of a SPO13 promoter–URA3 fusion reporter gene. Nucleotide numbering is relative to the translation start codon ATG, where A is +1. Red numbering represents nucleotides from the URA3 gene, and green represents those from the SPO13 gene. (B) Schematic representation of reporter yeast strains.
