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Cotransfection of boTLR2 with full length boTLR1 resulted in the recognition of triacylated LP. HEK293 cells were transfected with boTLR2 in combination with short boTLR1 (black bars) or full length boTLR1 (grey bars). Cells were stimulated for 24 h with 10 ng/mL TNF-α and 100 nM of the lipopeptides Pam2C-SK4 and Pam3C-SK4. IL-8 production was measured in the supernatant. Values are means ± SD of n = 2 and represent the result of at least 3 independent experiments.
