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. Author manuscript; available in PMC: 2010 Mar 3.
Published in final edited form as: Nature. 2009 Jun 10;460(7252):259–263. doi: 10.1038/nature08099

Figure 2. The lack of bone marrow adipocytes post-irradiation in fatless mice enhances hematopoietic progenitor expansion and post-transplant recovery.

Figure 2

a.Experimental design. Wildtype FVB or fatless FVB.A-ZIP/F 16-week-old mice (CD45.1) were lethally irradiated and transplanted with 200,000 CD45.2, MHC-compatible DBA/1 wild-type BM. Femurs were isolated on day 17–20 post-transplant and donor DBA CD45.2 wildtype BM was recovered by high purity FACS, then used for progenitor assays or competitive serial transplantation. b.Femoral H&E in the third week post-transplant. c.White blood cell (WBC) counts and d.hemoglobin levels in peripheral blood after primary transplant. BM recovered from primary transplants was assayed for e.relative frequency of progenitors by FACS (± STD) f.colony forming units assay (CFU), and g.secondary competitive transplantation into wildtype recipients.