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. Author manuscript; available in PMC: 2010 Mar 3.
Published in final edited form as: Biochem Biophys Res Commun. 2006 Jan 30;341(4):1257–1265. doi: 10.1016/j.bbrc.2006.01.092

Fig. 6.

Fig. 6

Overexpression of Osx in BMSC does not increase their chondrogenic or adipogenic potential. RCAS-Osx transduced BMSC and empty vector transduced BMSC and untransduced BMSC were cultured in chondrogenic induction media (A,B) or adipogenic induction media (C,D). (A) Levels of α1(II) procollagen, a chondrogenic differentiation marker were determined by RT-PCR and then normalized with those of the loading control GAPDH in three independent experiments. ap < 0.05 vs. untransduced BMSC or transduced with empty RCAS. Values at 4 weeks were significantly different (p < 0.05) vs. 1 week time point in the three groups. (B) Alcian blue stainings were performed to demonstrate chondrogenic differentiation at 4 weeks. (C) Levels of LPL, an adipogenic differentiation marker, were determined by RT-PCR and then normalized with those of the loading control GAPDH in three independent experiments. All values were significantly different (p < 0.05) vs. 1 week time point. (D) Oil Red O staining was performed to demonstrate adipogenic differentiation at 4 weeks.