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. 2010 Feb 16;123(6):861–870. doi: 10.1242/jcs.060475

Fig. 5.

Fig. 5.

GSK-3β suppression causes Bif-1 protein accumulation. (A,B) PC-3 cells were left untreated or treated with L803-mts at indicated doses (μM) in the presence or absence of 3-MA (10 mM) for 18 hours in serum-free medium. After harvesting, equal amounts of cellular proteins were subjected to SDS-PAGE and immunoblotting with the indicated antibodies. The relative band densities were normalized against the anti-actin blot and summarized in B. Data are from two separate experiments and error bars indicate the s.e.m. Asterisks indicate significant difference compared with the control (ANOVA, P<0.05). (C) PC-3 cells were treated with the solvent or L803-mts (100 μM) in serum-free medium. Cells were harvested at the indicated time points and Bif-1 protein levels were assessed by western blotting. (D) PC-3 cells were treated with the solvent, L803-mts (100 μM) or LiCl (10 mM) in serum-supplied (FBS, 10%) medium. Cells were harvested at the indicated time points and western blotting was conducted with the primary antibodies as indicated. The anti-actin blot served as a protein loading control.