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. 1970 Sep;67(1):128–135. doi: 10.1073/pnas.67.1.128

Biochemical and Genetic Studies of Recombination Proficiency in Escherichia coli, II. Rec+ Revertants Caused by Indirect Suppression of Rec-Mutations

Stephen D Barbour 1,2,*, Haruko Nagaishi 1,2, Ann Templin 1,2, Alvin J Clark 1,2
PMCID: PMC283177  PMID: 4248156

Abstract

All recB- and recC- mutants of E. coli carry out significant residual genetic recombination, whereas all recA- mutants form no recombinants. This observation suggests that an alternative minor pathway of recombination, independent of recB+ and recC+ products, may be operative in Escherichia coli. Rec+ revertants of recB- recC+, recB+ recC-, and recB- strains of E. coli have been isolated and are shown to fall into at least two major genotypic classes. One class carries revertant mutations which map in or very near the recB and recC genes. In this class an ATP-dependent DNase characteristic of wild type E. coli is restored. The reversions in this class are probably back-mutations or intragenic suppressor mutations. A second class carries revertant mutations which are located far from the recB and recC genes. In this class there is a high level of DNase activity which does not require ATP and is inactive on T4 DNA. Indirect and not informational suppression appears to be responsible for the second class of revertants. The suggestion is made that restoration of recombination by indirect suppression involves an activation or derepression of one or a series of enzymes, which participate in a pathway of recombination, alternative to the recB and recC pathway, but normally of minor importance. The ATP-independent DNase may be one of these enzymes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barbour S. D., Clark A. J. Biochemical and genetic studies of recombination proficiency in Escherichia coli. I. Enzymatic activity associated with recB+ and recC+ genes. Proc Natl Acad Sci U S A. 1970 Apr;65(4):955–961. doi: 10.1073/pnas.65.4.955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CLARK A. J. Genetic analysis of a "double male" strain of Escherichia coli K-12. Genetics. 1963 Jan;48:105–120. doi: 10.1093/genetics/48.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CLARK A. J., MARGULIES A. D. ISOLATION AND CHARACTERIZATION OF RECOMBINATION-DEFICIENT MUTANTS OF ESCHERICHIA COLI K12. Proc Natl Acad Sci U S A. 1965 Feb;53:451–459. doi: 10.1073/pnas.53.2.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. A proposal for a uniform nomenclature in bacterial genetics. Genetics. 1966 Jul;54(1):61–76. doi: 10.1093/genetics/54.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dürwald H., Hoffmann-Berling H. Endonuclease-I-deficient and ribonuclease I-deficient Escherichia coli mutants. J Mol Biol. 1968 Jul 14;34(2):331–346. doi: 10.1016/0022-2836(68)90257-x. [DOI] [PubMed] [Google Scholar]
  6. Emmerson P. T. Recombination deficient mutants of Escherichia coli K12 that map between thy A and argA. Genetics. 1968 Sep;60(1):19–30. doi: 10.1093/genetics/60.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gorini L., Beckwith J. R. Suppression. Annu Rev Microbiol. 1966;20:401–422. doi: 10.1146/annurev.mi.20.100166.002153. [DOI] [PubMed] [Google Scholar]
  8. Low B. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12. Proc Natl Acad Sci U S A. 1968 May;60(1):160–167. doi: 10.1073/pnas.60.1.160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Oishi M. An ATP-dependent deoxyribonuclease from Escherichia coli with a possible role in genetic recombination. Proc Natl Acad Sci U S A. 1969 Dec;64(4):1292–1299. doi: 10.1073/pnas.64.4.1292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Taylor A. L., Trotter C. D. Revised linkage map of Escherichia coli. Bacteriol Rev. 1967 Dec;31(4):332–353. doi: 10.1128/br.31.4.332-353.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Willetts N. S., Clark A. J. Characteristics of some multiply recombination-deficient strains of Escherichia coli. J Bacteriol. 1969 Oct;100(1):231–239. doi: 10.1128/jb.100.1.231-239.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Willetts N. S., Clark A. J., Low B. Genetic location of certain mutations conferring recombination deficiency in Escherichia coli. J Bacteriol. 1969 Jan;97(1):244–249. doi: 10.1128/jb.97.1.244-249.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Willetts N. S., Mount D. W. Genetic analysis of recombination-deficient mutants of Escherichia coli K-12 carrying rec mutations cotransducible with thyA. J Bacteriol. 1969 Nov;100(2):923–934. doi: 10.1128/jb.100.2.923-934.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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