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. Author manuscript; available in PMC: 2010 Mar 3.
Published in final edited form as: Science. 2009 Jul 24;325(5939):433. doi: 10.1126/science.1172447

Fig. 1.

Fig. 1

ZFN-mediated gene disruption in rat embryos. (A) ZFNs containing 5–6 fingers were designed to target coding sequences of interest (gray lines) for site-specific cleavage. (B) Two of five pups born after microinjection of GFP-targeted ZFNs were devoid of GFP expression. (C) Polymerase chain reaction using GFP-specific primers revealed truncated, but no wild-type sequence in each of the GFP negative pups compared to positive littermates. SS- Dahl S control DNA; NT indicates no template. (D) Table of injection data revealing successful mutagenesis of the three gene targets after multiple delivery methods and doses in three rat strains.