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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Radiat Res. 2010 Feb;173(2):175–183. doi: 10.1667/RR1982.1

FIG. 5.

FIG. 5

siRNA-directed knockdown of critical factors in homologous recombination (Rad51, Rad51C, Xrcc3) does not significantly change the effects of WR-1065 in human MCF-10A cells. Panel A: Western blot demonstrating knockdown efficiency. Panel B: Radio-protective effects of WR-1065 at 6 Gy. MCF-10A cells were treated with 4 mM WR-1065 for 30 min (gray bars) or sham-treated, irradiated with 6 Gy, and immediately replated for clonogenic survival. Data are normalized to sham-treated controls. Panel C: Cytotoxicity of WR-1065 in MCF-10A cells. siRNA-transfected cells (○, non-targeted siRNA;●, anti-Rad51;■, anti-Rad51C;◆, anti-Xrcc3) were treated continuously with WR-1065 (0–10 mM) for 7 to 10 days. Results represent means of three independent experiments ± SEM.