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. 2010 Feb 10;9:35. doi: 10.1186/1476-4598-9-35

Figure 2.

Figure 2

Extracellular matrix rigidity modulates neuroblastoma cell proliferation. (A) Phase contrast and fluorescence images of cell nuclei (DAPI) and Ki67 antibody staining (red), a marker of cell proliferation, of SK-N-DZ neuroblastoma cells on 0.1 kPa and 1000 kPa collagen-coated PA gels in the presence or absence of 1 μM 13-cis RA for three days. Scalebar = 50 μm. (B) WST-1 cell proliferation assay of SK-N-DZ neuroblastoma cells cultured on 1, 50, and 300 kPa collagen-coated PA gels with and without exposure to 1 μM 13-cis RA for three days. Error bars represent standard deviations of 5 technical replicates.