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A sequence located between -72 and -53 confers basal transcriptional activity of human LRP5 promoter. (A and B) Serial 5' deletion constructs were transfected into U2OS and HEK293 cells, and the relative luciferase activity was determined. The activity of pGL3-basic construct in A or that of pWT-359 construct in B was arbitrarily set to 100%, and the relative luciferase activity of the other constructs was calculated accordingly. Each bar represents the value of mean ± SEM.
