Table 1.
The oligonucleotide sequence of primers used in PCR
| Name | Sequence(5'→3') | Strand | Location |
|---|---|---|---|
| LRP5F | GCCTGACTGAGGAGCTGAAG | sense | -2381 ~ -2361 |
| LRP5R | GCTGCCTCCATGTTGTCC | antisense | +105 ~ +125 |
| LRP5R/HindIII | CCCAAGCTTGCTGCCTCCATGTTGTCC | antisense | +105 ~ +125 |
| -1901 F/KpnI | GACGGTACCAGCTACGATCACACCACTAC | Sense | -1901 ~ -1881 |
| -1594 F/KpnI | GACGGTACCGGCTGCCATGAGATCAGGTG | Sense | -1594 ~ -1574 |
| -1442 F/KpnI | GACGGTACCAGGCTGTGCTGGTAGAGCTG | sense | -1442 ~ -1422 |
| -1309 F/KpnI | GACGGTACCCTGTACCTTCTACCACCAGC | Sense | -1309 ~ -1279 |
| -1167 F/KpnI | GACGGTACCTTGGTAGCAGCTCAAGTGTC | Sense | -1167 ~ -1147 |
| -1085 F/KpnI | GACGGTACCTTCTTCATGGCCTGTCAATG | Sense | -1085 ~ -1065 |
| -953 F/KpnI | GACGGTACCAATCTGGAGGAACACACACG | Sense | -953 ~ -933 |
| -359 F/KpnI | GACGGTACCTGAAACTGCCTCGCTGTGTG | Sense | -359 ~ -339 |
| -219 F/KpnI | GACGGTACCGCCAAGCCTCCCGGAGGCAC | Sense | -219 ~ -199 |
| -167 F/KpnI | GACGGTACCCCGCGGGGACCTGCGCCGCT | Sense | -167 ~ -147 |
| -72 F/MluI | CGACGCGTGGCGGCGGGGGGCGGAGAAG | Sense | -72 ~ -52 |
| -53 F/MluI | CGACGCGTGCGGCGCGGCTTCCGCTCCCG | Sense | -53 ~ -33 |
| -40 F/KpnI | GACGGTACCCGCTCCCGCGCGCCCAGCTC | Sense | -40 ~ -20 |
| -72MT1/MluI | CGACGCGTGGCGGCTTTGGGCGGAGAAG | Sense | -72 ~ -52 |
| -72MT2/MluI | CGACGCGTGGCGGCGGGGGGTTGAGAAGCGGCG | Sense | -72 ~ -52 |
| -72MT12/MluI | CGACGCGTGGCGGCTTTGGGTTGAGAAGCGGCG | Sense | -72 ~ -52 |
Location: Nucleotide positions relative to the transcription start site (+1) of the human LRP5 gene.
Restriction sites are indicated in bold. The nucleotides of specific mutagenesis are indicated in italic and bold.