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Primers and TaqMan probes used to quantify mRNA expression of SIAH-1, Kid/KIF22 and β2 microglobulin.
| Gen | PCR Product (bp) |
Primers | TaqMan Probe |
|---|---|---|---|
| SIAH-1 | 75 | 5'-GCTAAATGGTCATAGGCGACG-3' 5'-ATGGCTGTTGCAATTCCTTCAT-3' |
5'-TTGACTTGGGAAGCGACTCCTCGATCTA-3' |
| Kid/KIF22 | 76 | 5'-CGGCCTTTTACCAATGAGAGC-3' 5'-GACCAAGCAATTCTTTCTGAGACA-3' |
5'-CAGCCTCATGCCTTGGGACCTGTTAAG-3' |
| β2 microglobulin | 67 | 5'-CGCTCCGTGGCCTTAGC-3' 5'-GAGTACGCTGGATAGCCTCCA-3' |
5'-TGCTCGCGCTACTCTCTCTTTCTG-3' |
