Figure 2.

ZONAB/Cold shock domain protein A is reexpressed in human kidney cancer. Relative mRNA levels were measured in six pairs of RCCs and corresponding normal tissue from the same kidney (n = 3) or matched normal kidney (n = 3) using qRT-PCR by reference to β-actin, then normalized to the corresponding ratio in nontumoral kidney, set as 100% (dotted line). Results are presented as plots showing 25th and 75th percentiles, medians (horizontal lines), and minimal and maximal values (vertical bars). All values in cancer samples are statistically different from controls by the nonpaired Wilcoxon ranking test, except for cubilin. Note that Cold shock domain protein A overexpression in carcinomas is associated with increased expression of the early proliferation regulator cyclin D1 and decreased expression of the apical differentiation markers megalin, villin, and presumably cubilin. For a meta-analysis of published microarray data, see Supplemental Figure S2.