Figure 9.

Glomerular eluates from MN patients contain antibodies against AR and SOD2. (A) Glomeruli showed presence of specific IgG₄ antibodies against AR and SOD2. IgGs were also present at a lower titer. Controls were incubated with human serum albumin (HSA) instead of glomerular eluates. (B) Corresponding bar graph shows dot-spot OD (in arbitrary units) for anti-AR (■) and anti-SOD2 (□) specific IgG₄. (C) Competition experiment using the same glomerular eluates as in A and increasing amount of r-AR and r-SOD2 from 5 to 15 ng.