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Water-deprived KO and WT mouse kidneys were immunostained for AQP2 and pS256-AQP2. AQP2 labeling in the CDs of inner medulla (A) or cortex (B) of WT mice shows intense labeling and apical localization compared with the inner medulla (C) or cortex (D) of KO mice. pS256-AQP2 labeling of inner medulla (E) or cortex (F) of WT mice shows apical labeling, whereas in the inner medulla (G) or cortex (H) of KO mice, labeling is less intense and more cytoplasmic. Original magnification, ×40.
