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Renal cAMP levels are reduced in KO mice. (A) cAMP levels were measured in the urine of WT and KO mice that were water deprived for 18 hours. $P < 0.05. (B) cAMP levels in renal papillae. Renal papillae were isolated, finely minced, and treated for 20 minutes with IBMX followed by dDAVP (1 μM) or forskolin (1 μM) for an additional 10 minutes. @P < 0.01; *P < 0.001; WT versus KO mice; n = 8.
