Figure 8.

GSK3β gene deletion or inhibition reduced adenylate cyclase activity. (A) Adenylate cyclase activity in response to dDAVP was determined in the intact membrane preparations obtained from renal inner medulla and papillae of WT or KO mice (*P < 0.001 WT versus KO mice treated with dDAVP; n = 5) or (B) forskolin (P < 0.0001; WT versus KO mice, determined by ANOVA; n = 5). (C) Adenylate cyclase activity in the membrane preparation of the renal papillae of WT mice treated with GSK3β inhibitor SB216763. (D) Adenylate cyclase 3 or 5/6 expression in renal medullary lysates of water-deprived WT and KO mice.