Figure 9.

Expression of other EMT markers in HK-2 cells overexpressing miR-192 by qRT-PCR is shown. Stable lines overexpressing miR-192 or a control short hairpin (Scrambled) were growth-arrested in serum-free medium for 48 hours before incubation with 10 ng/ml TGF-β (■) or control medium (□) for 96 hours. (A through F) Expression of PAI-1 (A), vimentin (B), Snail (C), Slug (D), Twist (E), and HMGA2 (F) was examined by qRT-PCR. The expression was normalized to GAPDH. Error bars represent SEM (n = 3).