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Transfection of HCV-hyg-replicons into Huh7, Huh7D, and Huh7.5 cells and selection with hygromycin B at a concentration of 65 μg/ml. A. Relative quantification of surviving colonies. B. Detection of HCV antigens in surviving Huh7D and Huh7.5 cells at 37 days post transfection by immunfluorscence.
