Table 1.
Reactivity of mAbs MEST-1, -2 and -3, with different fungi preparation
| MEST-1 Galfβ1→3/6Manp |
MEST-2 GlcCer |
MEST-3 Manpα1→3Manpα1→2Ins |
|||||
|---|---|---|---|---|---|---|---|
| HPTLC | IFI | HPTLC | IFI | HPTLC | IFI | ||
| Pb | Y | + | + | + | + | + | + |
| M | + | - | + | - | + | - | |
| Ss | Y | - (np) | - (np) | + | + | + | + |
| M | - (np) | - (np) | + | - | - (np) | - (np) | |
| Hc | Y | + | + | + | + | + | + |
| M | - (np) | - (np) | + | - | - (np) | - (np) | |
Reactivity of mAbs MEST-1, -2 and -3, with fungal glycolipids by HPTLC immunostaining (HPTLC); and with fixed fungi by indirect immunofluorescence (IFI). Pb = P. brasiliensis; Ss = S. schenckii; Hc = H. capsulatum; Y = yeast; M = mycelium; MEST-1 recognizes epitope Galfβ1→3/6Manp; MEST-2 recognizes fungal glucosylceramide (GlcCer); MEST-3 recognizes epitope Manpα1→3Manpα1→2Ins; "+" indicates positive staining; "-" indicates negative staining, and "np" indicates epitope not present. [13,24].