Figure 2. The volume of the Golgi is increased following Scyl1 knock down.

A, HeLa cells transfected with control (ctrl) or Scyl1 #1 siRNA were stained for GM130. Z-series of complete Golgi stacks were imaged by confocal microscopy and volume surface rendered using Imaris software as described in Materials and Methods. The scale bar = 10 µm. The volume of the Golgi labelled with antibody against GM130 was determined as described in Materials and Methods. A significant increase (*** p<0.001; Student's t-test) in Golgi volume was seen, as indicated graphically on the right. B, HeLa cells transfected with control or Scyl1 siRNA were stained for βCOP and processed as described in A. C, HeLa cells transfected with p115 siRNA were stained for GM130. Z-series of complete Golgi stacks were imaged by confocal microscopy and volume surface rendered using Imaris software. The scale bar = 10 µm. Error bars represent standard deviation.