Figure 7. Scyl1 has several properties of golgins.

A, P3 microsomes, enriched in Golgi membranes, were prepared from brain extracts and were resuspended in 10 mM HEPES, pH 7.4 or the same buffer containing 150 mM NaCl or 1% Triton X-100, or in Na-carbonate buffer at pH 11. The samples were then spun at high g and the supernatant (S) and pellet (P) fractions were analyzed by Western blot with the indicated antibodies. B, GFP-Scyl1 was transfected into HeLa cells and the Golgi area was bleached. The recovery of fluorescence on the Golgi was recorded over time.