Figure 8. Scyl1 binds 58K.
A, GST-tagged full-length Scyl1 (GST-FL) or the isolated C-terminal region (GST-CT), along with GST alone were incubated with rat liver extracts and specifically bound proteins were processed for Western blot with antibodies against Na/K ATPase and 58K. B, Schematic representation of Scyl1 full-length (FL) and the full-length protein lacking the last 10 amino acids (Δ10). C, The constructs described in B were used in affinity selection assays followed by Western blot with antibodies for Na/K ATPase, 58K and βCOP. For A and C, starting material (SM) represents 1/20 of the input to the selection experiment.