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. 2010 Mar 4;5(3):e9537. doi: 10.1371/journal.pone.0009537

Figure 9. Disruption of the golgin network displaces Scyl1 from the Golgi.

Figure 9

A, HeLa cells were transfected with control or p115 siRNA and were subsequently fixed and processed for indirect immunofluorescence with antibodies against Scyl1 (red) and GM130 (green). The area indicated by the box in the merged image is shown at higher power on the right. Arrowheads indicate co-localizing structures. The scale bar = 10 µm for the low and 1 µm for high power images, respectively. B, HeLa cells were transfected with control or p115 siRNA and with ERGIC53-YFP, and were subsequently fixed and processed for indirect immunofluorescence with antibodies against Scyl1 (red) and GM130 (blue) with ERGIC53 imaged in the green channel; top eight panels or with antibodies against TGN46 (red), GM130 (green) and Scyl1 (blue); bottom 4 panels. For the middle four panels, arrowheads indicate co-localizing structures. For the bottom four panels, arrowheads indicate areas where TGN46 and GM130 are found in close apposition and lack the presence of Scyl1. The scale bar = 1 µm.