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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Genes Immun. 2009 Oct 15;11(2):161–172. doi: 10.1038/gene.2009.73

Figure 2. 5′ deletion analysis of hFli1 promoter activity in human T cells.

Figure 2

Figure 2

(A) Schematic of the human Fli1 −1766 to −37 region. Deletions were made from the 5′ end of the pGL3 human Fli1 −271/−37 as indicated to generate a series of promoter/reporter constructs. (B) Transient transfection of constructs into the Jurkat human T cell line. Expression is presented relative to the pGL3 Basic empty vector, which was set to 1. (C) Transient transfection of pGL3 human Fli1 −271/−37 containing mutations in each of the putative transcription factor binding sites indicated in Fig. 1B. Results are representative of at least three independent transfections performed with two independently derived clones.