Figure 3.

Stairway sequential assignment of GB1 with the CANCA experiment recorded at conditions simulating a 90 kDa protein (20% glycerol, 285K). Red crosses indicate the correlations expected from square pattern in C^α-C^α plane. Blue arrows indicate the assignment walk in the “stairway” procedure from the C^α of residue 19 to the N of residue 23 (from top to bottom). Starting from C^α_i in a C^α-C^α plane at ω (N_i), one can find the nitrogen frequency of the preceding residue ω (N_i+1) at the C^α_i cross section in a C^α-N plane (strips on the side). The C^α-C^α plane at ω (N_i+1) gives the assignment of C^α_i+1. Slices at the position of horizontal broken lines are shown in C^α-C^α planes.