. Author manuscript; available in PMC: 2011 Apr 1.

Published in final edited form as: Cancer Lett. 2009 Sep 16;290(1):49–57. doi: 10.1016/j.canlet.2009.08.025

Table I.

Mass spectroscopy identification of a MIF complex in the 2D gel band.

Fraction 5	Serpinc1 Serine peptidase inhibitor, clade C (Antithrombin), member 1
Peptide	Accession #	Protein Identification Probability	Peptide sequence	Mascot Identity Score	Calculated Peptide Mass (daltons)
1	IPI00372372	100%	EQLQDMGLVDLFSPEK	31.5	1864.9006
2	IPI00372372	100%	LQPLDFK	34.6	860.4883
3	IPI00372372	100%	NDNDNIFLSPLSISTAFAMTK	30.5	2315.1228

Fragment ion data generated by Information Dependent Acquisition via QSTAR ® were searched against National Center for Biotechnology Information nr sequence database using Mascot (Matrix Science, Boston, Massachusetts) database search engine. Probability based MOWSE (Molecular Weight Search) scores above default significant value were considered for protein identification, in addition to validation by manual interpretation of tandem MS data. Column 1, peptide number. Column 2, National Center for Biotechnology Information reference number for identified protein. Column 3, probability of accurate protein identification. Column 4, peptide sequence. Column 5, Mascot identity score. Probability is defined as the observed database match is random event. An individual score >30 indicates identity or extensive homology, Column 6, calculated peptide mass.