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. Author manuscript; available in PMC: 2011 Mar 10.
Published in final edited form as: J Am Chem Soc. 2010 Mar 10;132(9):2983–2990. doi: 10.1021/ja908395r

Table 1.

Properties of lumazine derivatives.

Sample pKa Absorbance maxima, nm Isosbestic points, nm

pH 6.9 pH 11.0
1 (aqueous solution) 7.9 407 312 346
1 in complex with wild type riboflavin synthase 8.0 413 318, 370 378
1 in complex with riboflavin synthase mutant T50A 10.1 409 317, 376 369
1 in complex with riboflavin synthase mutant T67A 8.9 418 315, 375 375
6 (aqueous solution) 9.8 401 311, 360 369