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. 2010 Mar;176(3):1157–1168. doi: 10.2353/ajpath.2010.090200

Figure 5.

Figure 5

Arhgef1-deficient peritoneal elicited macrophages exhibit increased integrin-mediated MMP expression. A: Adhesion of wild-type (open bars) and Arhgef1−/− (solid bars) PEMs to BSA, laminin, ICAM-1, VCAM-1, and fibronectin after 48 hours of incubation. Wild-type (n = 6) and Arhgef1−/− (n = 6) from 3 independent experiments. B: MMP2 (top), MMP9 (middle), and MMP12 (bottom) expression measured by qPCR of wild-type (open bars) and Arhgef1−/− (solid bars) PEMs cultured for 48 hours on the indicated substrates. MMP expression is shown as fold relative to wild-type samples cultured on BSA with all samples normalized to 18s RNA. Wild-type (n = 8) and Arhgef1−/− (n = 12) from 4 independent experiments. C: MMP activity as quantified by densitometric analysis of conditioned media from macrophages cultured on fibronectin, wild-type (open bars; n = 6), and Arhgef1−/− (solid bars; n = 6). Data represent mean ± SE. *P < 0.05 Student two-tailed t test compared with cells on BSA. **P < 0.05 Student two-tailed t test comparing wild-type to Arhgef1−/− cells.