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. 2010 Mar;176(3):1421–1432. doi: 10.2353/ajpath.2010.090438

Figure 2.

Figure 2

Loss of heterozygosity during mammary tumorigenesis in BALB/c-Trp53+/− mice. The proportions of wild-type (WT) and null (NULL) alleles in tumors were determined by Southern blot hybridization and were compared with the ratios for tail DNAs from mice that were homozygous for the wild-type allele or heterozygous (Trp53+/+ and Trp53+/−, respectively). Reductions in the wild-type allele below 50% (indicated by the dashed line) were considered significant loss of heterozygosity. The transplanted ductal hyperplasias (PH1b and PH2) used for transplantation retained the wild-type allele at levels equivalent to tail DNA controls. However, tumors developing from the hyperplasias showed severe loss of heterozygosity in all cases except for MT6577.