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. 2010 Mar;176(3):1452–1461. doi: 10.2353/ajpath.2010.090523

Figure 6.

Figure 6

KLK4 activates p42/p44 MAPK in HT29 cells. A: Immunoblot with phospho-specific p42/p44 MAPK antibodies on quiescent HT29 cell lysates treated with or without KLK4 (0.5 μmol/L) or thrombin (0.01 μmol/L) for the indicated time periods (shown in minutes). B: Dose-dependent activation of p42/p44 MAP-kinase phosphorylation by KLK4. Quiescent HT29 cells were stimulated with the indicated concentrations of KLK4 or with thrombin (0.01 μmol/L) for 10 minutes. To confirm equal protein loading, the membranes were stripped and incubated with p42/p44 antibodies. Results are representative of two independent experiments.