Figure 2.

ADAMTS9 is expressed in microvascular endothelial cells (ECs) and aortic mural cells during mouse embryonic angiogenesis and in adult mice. All images show combined β-gal histochemistry (blue) and PECAM-1 (A, B, red) or endomucin (C−F, red) immunohistochemistry from 9.5-day embryos (A, B), 11.5-day umbilical cord (C), 13.5-day mouse embryos (D−F) and adult tissues (G−L). Arrows indicate endomucin+, β-gal+ cells. The asterisks in A, D, H, and I indicate a large blood vessel. A: ECs of mesenchymal capillaries (arrows) posterior to the dorsal aorta (asterisk), but not ECs lining the aorta are β-gal+. A β-gal+ putative mural cell is indicated by an arrowhead. B: β-gal−stained ECs in forebrain. C: ECs of the umbilical vein (UV), but not the arteries (UA) are β-gal+. Note that β-gal is strongly expressed in mural cells of umbilical vessels. D: β-gal+ ECs are seen in capillaries (arrows) arising from a larger blood vessel (asterisk), but not the ECs lining the large vessel (asterisk). E: ECs lining somitic capillaries are β-gal+. F: High power view of a capillary from E13.5 spinal cord illustrates that β-gal + nuclei line the capillary lumen and are surrounded by endomucin+ cytoplasm. G: Adult myocardial capillaries all contain β-gal+ ECs (inset: myocardial capillary at higher magnification). H: Adult aorta. Arrowhead indicates β-gal+ SMC in the tunica media. Note the absence of β-gal staining in ECs (arrow). I: Perirenal fat. Note the absence of β-gal staining in large vessel ECs (asterisk) whereas every capillary ECs express β-gal. J: Thymus. Note that in addition to EC (arrows), some non-EC thymic cells are also β-gal positive. K: In the renal cortex, the glomerular tuft (G) does not express β-gal. L: Section through the bed of a 5-day-old excisional skin wound. EC (arrows) invading the thrombus are β-gal+. Inset shows a β-gal+ stained tip cell invading the thrombus. Scale bars: 37 μm (A−E, G, I) and 11 μm (F, H, J, K, L).