Skip to main content
. 2010 Mar;176(3):1494–1504. doi: 10.2353/ajpath.2010.090655

Figure 7.

Figure 7

ADAMTS9 does not cleave thrombospondin-1 and -2. A: Analysis of TSP-1 cleavage using Western blotting with an anti-TSP-1 antibody under reducing and nonreducing conditions. The TSP-1 fragment released by ADAMTS1-transfected cells is indicated by the arrowheads. The panels at the bottom show Western blotting with anti-FLAG M2 or anti-ADAMTS1 monoclonal antibody to verify expression of the transfected ADAMTS plasmids. B: Analysis of TSP-1 cleavage using the neoepitope antibodies 78 and 79 and the cleavage-site−spanning antibody 80. The arrowheads indicate the ADAMTS1 cleavage fragments. C: Analysis of TSP-2 cleavage: The arrowhead indicates the ADAMTS1 cleaved fragment. The panels at the bottom show Western blotting with anti-FLAG M2 or anti-ADAMTS1 monoclonal antibody to verify expression of the transfected ADAMTS plasmids.