Table 1.

Primer Sequences Used for qRT-PCR Analysis

Name	Forward primer	Reverse primer
β-Actin	5′-GGCTGTATTCCCCTCCATCG-3′	5′-CCAGTTGGTAACAATGCCATGT-3′
TNF-α	5′-CCCTCACACTCAGATCATCTTCT-3′	5′-GCTACGACGTGGGCTACAG-3′
IL-1β	5′-GCAACTGTTCCTGAACTCAACT-3′	5′-GGTCAAAGGTTTGGAAGCAG-3′
IL-1Ra	5′-CTGATGAAGCTCGTCAGGCAG-3′	5′-TGGTGCTGAGATAGTGTTTGTC-3′
M. pulmonis 16S rRNA	5′-CGGTACAGGAAACTGTTGCTAATACCG-3′	5′-CCATTTCAAAGTGAAGCAAACG-3′
S100A8	5′-CCGTCTTCAAGACATCGTTTGA-3′	5′-GTAGAGGGCATGGTGATTTCCT-3′
Iba1	5′-GCTGGAGGGGATCAACAAGC-3′	5′-TCTTCAGCTCTAGGTGGGTCT-3′
CD19	5′-AGTGATTGTCAATGTCTCAGACC-3′	5′-CCCCACTATCCTCCACGTTCA-3′
CD3e	Custom primers from SABiosciences (Frederick, MD)	Custom primers from SABiosciences
VEGF-A	5′-ATCTTCAAGCCGTCCTGTGT-3′	5′-GCATTCACATCTGCTGTGCT-3′
VEGF-C	Custom primers from SABiosciences	Custom primers from SABiosciences
VEGF-D	5′-TTGAGCGATCATCCCGGTC-3′	5′-GCGTGAGTCCATACTGGCAAG-3′
Angiopoietin-1	5′-AGCTACCAACAACAACAGCA-3′	5′-GCAAAGGCTGATAAGGTTATGA-3′
Angiopoietin-2	5′-GTCACAATGACAAGGCCGTG-3′	5′-TCCCTGGATACTGGCTGCC-3′
PDGF-B	5′-GATCTCTCGGAACCTCATCG-3′	5′-GGCTTCTTTCGCACAATCTC-3′