Figure 1.

(a) In situ click chemistry reactions between an anchor molecule, acetylenic benzenesulfonamide and a library of 20 complementary azides in the presence of a target enzyme bCAII. (b) Schematic representation of the 1^st-generation screening microreactor for testing the feasibility to perform a small scale screening of 32 in situ click chemistry reactions. The responsibilities of different hydraulic valves are illustrated by their colors: red for isolation valves and yellow for pump valves (for fluidic metering and circulation). (c) Optical image of the actual device. The various channels were loaded with food dyes to help visualize the different components of the microfluidic chip; the colors correspond to those in b), with blue indicating the fluidic channels.