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Summary of the comparison among the conventional 96-well approach and the two generations of screening microreactors.
| Number of reactions |
Enzyme (bCAII) (µg) |
Alkyne (nmol) |
Azide (nmol) |
Total reaction volume (µL) |
Sample preparation time |
Hit identification time |
Detction methods |
|
|---|---|---|---|---|---|---|---|---|
| 96 well | 96 | 94 | 6 | 40 | 100 | few mins | few mins | LC-MS |
| 1st-Generation | 32 | 19 | 2.4 | 3.6 | 4 | 58 sec | 58 sec | LC-MS |
| 2nd-Generation | 1024 | 0.36 | 0.12 | 0.12 | 0.4 | 15 sec | 15 sec | MRM |
