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. Author manuscript; available in PMC: 2010 Aug 7.
Published in final edited form as: Science. 2009 Aug 7;325(5941):753–756. doi: 10.1126/science.1172221

Fig. 1. Protein translocation in E. coli.

Fig. 1

(A) Jamming of SecY occurs during post-translational translocation of the LamB-LacZ hybrid, resulting in accumulation of precursor proteins such as pMalE (in red) with their signal sequence (in green) still attached. (B) When the hybrid is targeted for co-translational translocation (H*LamB-LacZ), it is secreted into the periplasm. Properties of the LacZ fusion strains are summarized in the text box. (C) Co-translational translocation of a wild-type SRP substrate (purple). (D) During co-translational translocation, antibiotics that inhibit peptide chain elongation jam the translocator. Orange circles; translating ribosome, OM; outer membrane, IM; inner membrane, IMP; IM protein, P; periplasm.