Skip to main content
. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Dev Biol. 2009 May 9;332(1):104–115. doi: 10.1016/j.ydbio.2009.05.541

Figure 3. Loss of E2F4 aggravates the placental defect in Rb−/− embryos at E11.5.

Figure 3

A) Control yolk sacs are highly vascularized while there is a decrease in the number of blood vessels in the Rb mutant yolk sacs. Loss of E2F4 further impairs vascularization in the yolk sac.

B) Hematoxylin and eosin stained placental sections of control and mutant littermates at a magnification of 10x, the bar in each panel represents 100µm. In Rb−/−;E2f4−/− placentas the height of the labyrinth layer is reduced. C) In situ hybridization for 4311 (Tpbp) indicates that the spongiotrophoblast layer (Sp) is relatively unaffected in the mutant placentas. Magnification, 10x. D) Trophoblast giant cell differentiation is largely unaffected in mutant placentas as assessed by in situ hybridization for placental lactogen-1. Magnification, 2x. *Position of trophoblast giant cells; MD, maternal decidua; C, chorionic plate; ET, embryonic tissue.