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. 2009 Dec 9;29(49):15551–15563. doi: 10.1523/JNEUROSCI.3336-09.2009

Figure 6.

Figure 6.

Hyperdopamine induced by systemic administration of GBR12909 significantly increases GSK-3β expression in the PFC. A, Analysis by Western blots showed that a single dose of GBR12909 (10 mg/kg, i.p.) caused significant increase of total protein levels of GSK-3β as well as the levels of the phosphorylated form of GSK-3β Tyr216 but not GSK-3β Ser9 at 1 h after injection. B, Quantitative analysis showed that the total protein levels of GSK-3β were significantly increased by ∼40% after GBR12909 administrations relative to saline vehicle control (p < 0.01). A similar increase of ∼30% was observed in the levels of phosphor-GSK-3β Tyr216 relative to saline vehicle control (p < 0.05). In contrast, the levels of phosphor-GSK-3β Ser9 remained unchanged compared with control levels (p = 0.67) and but were significantly lower compared with total protein levels of GSK-3β (p < 0.01). Error bars indicate SEM.