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. Author manuscript; available in PMC: 2010 Mar 4.
Published in final edited form as: Mol Cell Pharmacol. 2009;1(1):23–28. doi: 10.4255/mcpharmacol.09.04

Figure 2. TG induced cytochrome c release from mitochondria into cytosol in Smac-proficient and -deficient cells.

Figure 2

Smac-proficient (Smac+/+) or Smac-deficient (Smac−/−) cells were left untreated or treated with 75 nM TG for approximately 24 h. Cytosolic fractions were prepared as we have previously described (28) and Western blotting was performed using the anti-cytochrome c antibody or the anti-Smac antibody. The same blots were later probed with the anti-β-actin antibody to detect β-actin as a loading control.