Figure 6.

Northern-blot analysis of transgenic P. patens plants expressing MpABI1 and MpABI1-d2. Total RNAs extracted from nontransgenic (NT) and independent transgenic lines expressing intact MpABI1 (W-1 to W-6) and MpABI1-d2 (D2-1 to D2-4) were electrophoresed and blotted onto a nylon membrane. The blotted RNAs were hybridized with ³²P-labeled probes for either the N-terminal domain region (N) or the C-terminal domain region (C) of MpABI1 cDNA. Ethidium bromide-stained rRNA for each sample is also shown.