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. 2010 Jan 22;152(3):1529–1543. doi: 10.1104/pp.110.153387

Figure 8.

Figure 8.

Freezing damage of nontransgenic (NT) and representative transgenic lines expressing different levels of transcripts of MpABI1 (W2, W3, and W4) and MpABI1-d2 (D2-1 and D2-2). Protonema cells were incubated for 1 d with or without 10 μm ABA and subjected to freezing. The cells were then ice inoculated at −1°C and subjected to equilibrium freezing at a rate of −2.4°C h−1 to desired temperatures. After thawing, electrolyte leakage was measured and survival was estimated as described previously (Nagao et al., 2005). A and B, Comparison of electrolyte leakage (%) of unfrozen and frozen plants that had been pretreated with 10 μm ABA for 1 d prior to freezing. A, Comparison of nontransgenic, W2, W3, and W4 plants. B, Comparison of nontransgenic, D2-1, and D2-2 plants. C and D, Nontreated (C) or 10 μm ABA-treated (D) protonema cells of nontransgenic, W-2, and D2-1 plants were frozen to −3°C, −6°C, and −9°C, and survival (%) after thawing was estimated by electrolyte leakage measurement.