Figure 9.

Analysis of ABA-induced soluble proteins in control (nontransgenic [NT]) and transgenic plants, W-2 and D2-1. Total soluble proteins (left) and boiling-soluble proteins (right) were subjected to SDS-PAGE and stained with Coomassie Brilliant Blue. Positions of molecular mass markers are shown in kD. B, The boiling-soluble proteins were used for immunoblot analysis after electroblotting onto an Immobilon-P membrane. The blotted proteins were reacted with antiserum raised against the recombinant 17B9 protein. Signals were detected using an alkaline phosphatase-conjugated secondary antibody and nitroblue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate color-developing reagent.