NaMYB8 regulates the accumulation of CP and DCS in M. sexta-attacked leaves. M. sexta caterpillars were allowed to feed on N. attenuata wild-type (WT) and NaMYB8 transgenic plants for 4 d before harvesting samples for analysis. A, HPLC chromatograms obtained from wild-type and ir-MYB-810 M. sexta-fed rosette leaf methanolic extracts detected at 254-nm wavelength. B to G, Mean ± se concentrations of CP (B), DCS (C), CGA (D), rutin (E), nicotine (F), and DTGs (G) in wild-type, ir-MYB8-818, and ir-MYB8-810 leaves directly attacked by herbivores quantified by HPLC. Asterisks represent significant differences among the genotypes within the treatment group at P < 0.001 (n = 5). FM, Fresh mass; nd, not detected.