Figure 6.

Flow rate through a stem explant flushed with deionized water. A 2-mL pulse of gold particle suspension was added 0.7 h after the beginning of the experiment. Water flushing was then continued for an additional 0.67 h, and then the water in the reservoir was replaced with 0.05 m CDTA solution, buffered at pH 6.0. The addition of CDTA did not change significantly the flow rate through the stem. Uronic acids were found in the eluent collected immediately after the CDTA addition. Colloidal gold was found in the eluting fluid fractions collected subsequent to the CDTA introduction and the detection of uronic acids in the eluent.