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. Author manuscript; available in PMC: 2011 Mar 12.
Published in final edited form as: J Mol Biol. 2009 Dec 21;396(5):1295–1309. doi: 10.1016/j.jmb.2009.12.017

Figure 5. Quantitative analysis of intramolecular interfaces in Ac-Nt17-QN-Nme constructs.

Figure 5

Panel A shows the length dependence of the enhancement / depletion in the probability of observing intramolecular inter-residue contacts between specific residues classes in monomers of Ac-Nt17-QN-Nme constructs. Results are shown for 305 K. The definition of a contact and the calculation of a random prior model are described in Methods. Panels B and C show the volumetric, C1(T), and surface energy contributions C2(T), for polyglutamine domains in Ac-Nt17-QN-Nme and homopolymeric constructs, respectively. The energetic terms were obtained from fits to the linear regression equation shown in the text. Fits were obtained using results for chain lengths N=(15, 20, 25, 30, 35, 45). The quality of the fits is assessed through the plotted errors bars, which result from a propagation of error though the fitting procedure. Only chains of length N≥15 are used, as shorter chains are not likely to have a volumetric contribution to the potential energy.