Figure 4.

Inhibition of cleavage by single or double bp substitutions and by the tRNA^Sec AD. (A) Relative cleavage rates of internally ³²P-labeled R1.1[WC] RNA variants exhibiting single or double W-C bp substitution in the distal or proximal box. The proximal box (PB) and distal box (DB) sequences of R1.1[WC] RNA are shown on the left. (B) Inhibition by the tRNA^Sec AD and sequence variants. The var-1 RNA value is from Fig. 2. (A and B) The numbers below the variant RNAs are cleavage rates, relative to that of R1.1[WC] RNA (100%), and represent the average of three experiments, with SDs averaging ≤22% of the values.