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. Author manuscript; available in PMC: 2010 Jun 16.
Published in final edited form as: Neuroscience. 2009 Mar 11;161(1):148–156. doi: 10.1016/j.neuroscience.2009.03.006

Figure 3. NGF in culture media does not change the stimulated release of iCGRP and the GDNF-induced enhancement in release.

Figure 3

Peptide release elicited by a 10 minute exposure to HEPES buffer alone (open bars) or HEPES buffer containing 50 nM capsaicin (Cap; dark bars) is expressed as mean percent total peptide content of cells in each well ± SEM (n = 9 wells per condition). 10 ng/mL GDNF was included in the 10 minutes prior to and throughout capsaicin exposure. Total growth factor exposure time was 20 minutes. NGF was added in the culture media at a concentration of 30 ng/mL or omitted from the culture media. Asterisks (*) indicate statistically significant differences in iCGRP release between GDNF treatment group and the no GFL condition using an t-test (p<0.05). In all cases, release stimulated by capsaicin was significantly higher than basal release.