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. Author manuscript; available in PMC: 2010 Jun 16.
Published in final edited form as: Neuroscience. 2009 Mar 11;161(1):148–156. doi: 10.1016/j.neuroscience.2009.03.006

Figure 4. GFLs do not enhance the potassium-stimulated release of iCGRP from isolated sensory neurons.

Figure 4

Peptide release elicited by a 10 minute exposure to HEPES buffer alone (open bars) or HEPES buffer containing 50 mM potassium (KCl; dark bars) is expressed as mean percent total peptide content of cells in each well ± SEM (n = 9-12 wells per condition). GDNF, neurturin (NTN), or artemin (ART) at 10 ng/mL, was included in the 10 minutes prior to and throughout potassium exposure. PGE2 was present in the basal and stimulated conditions at a concentration of 500 nM. Total growth factor and PGE2 exposure time was 20 minutes. There were no significant differences in iCGRP release between treatment groups and the no GFL condition. A significant enhancement in iCGRP release was observed with PGE2 using ANOVA with Dunnett's post-hoc test (p<0.05). In all cases, release stimulated by potassium was significantly higher than basal release.